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Influence of Controlled- and Uncontrolled-pH Operations on Recombinant Phenylalanine Ammonia Lyase Production in Escherichia coli

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 4 (2009.08)바로가기
  • 페이지
    pp.954-958
  • 저자
    Jian Dong Cui, Gui Xia Zhao, Ya Nan Zhang, Shi Ru Jia
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A110373

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원문정보

초록

영어
Effects of controlled- and uncontrolled-pH operations on phenylalanine ammonia lyase (PAL) production by arecombinant Escherichia coli strain were investigated at uncontrolled-pH (pHUC) and controlled-pH (pHC) of 5.5, 6.0, 6.5, 7.0,7.5, 8.0, and 8.5 in bioreactor systems. The results showed that the recombinant PAL activity was improved significantly bycontrolled pH strategy. Among the pHC operations, the highest PAL activities were obtained under pHC 7.5 strategy where cellmass (OD600 nm) and PAL activity was 1.3 and 1.8 fold higher than those of pHUC, respectively. The maximum PAL activityreached 123 U/g. The pHC 7.5 strategy made recombinant plasmid more stable and therefore allowed easier expression of PALrecombinant plasmid, which increased PAL production. It was indicated that the new approach (controlled-pH strategy)obtained in this work possessed a high potential for the industrial production of PAL, especially in the biosynthesis of L-phenylalanine.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

enzyme activity recombinant Escherichia coli controlled-pH strategy phenylalanine ammonia lyase fermentation

저자

  • Jian Dong Cui [ Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhang 050018, PR China ] Corresponding author
  • Gui Xia Zhao [ Labour Union, Hebei University of Science and Technology, Shijiazhang 050018, PR China ]
  • Ya Nan Zhang [ Library, Hebei University of Science and Technology, Shijiazhang 050018, PR China ]
  • Shi Ru Jia [ Key Laboratory of Industry Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tai Da Development Area, Tianjin 300457, PR China ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 18 Number 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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