A-Rang Im, Joon-Soo Sim, Youmie Park, Bum-Soo Hahn, Toshihiko Toida, Yeong Shik Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A110359
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원문정보
초록
영어
By-products of marine organisms including salmon, skate, flatfish, and yellow goosefish were investigated tosearch for new source of chondroitin sulfate (CS). Agarose gel electrophoresis with chondroitinase depolymerization showedthat purified chondroitin sulfate did not contain any other glycosaminoglycans. 1H-nuclear magnetic resonance (NMR) spectrawere acquired to confirm the structure and purity. The average molecular weight ranging from 22 to 64 kDa was determinedby high performance size exclusion chromatography. Disaccharide compositions and purities were determined by strong anionexchange-high performance liquid chromatography (SAX-HPLC) after chondroitinase ABC depolymerization. SAX-HPLCdata exhibited that the purity was from 81.7±1.3 to 114.2±2.5% and the yield was from 1.3 to 12.5%. All analytical resultsindicate that salmon cartilage, skate cartilage, and yellow goosefish bone could be promising sources of CS to substitute sharkcartilage CS in commercial neutraceuticals.
목차
Abstract Introduction Materials and Methods Results and Discussion References
A-Rang Im [ Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-741, Korea ]
Joon-Soo Sim [ Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-741, Korea; National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi 441-707, Korea ]
Youmie Park [ Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-741, Korea ]
Bum-Soo Hahn [ National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi 441-707, Korea ]
Toshihiko Toida [ Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 263-8522, Japan ]
Yeong Shik Kim [ Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-741, Korea ]
Corresponding author