Response surface methodology (RSM) was used for the optimization of antioxidant capacity in gardenia extracts. Theantioxidant capacities of gardenia fruit extracts were investigated by ferric reducing ability (FRA) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging activity (RSA) assays. The optimum extraction parameters for the strongest antioxidantcapacity were the ethanol concentration (EtOH) of 48.9%, extraction temperature of 72.9oC, and extraction time of 29.9 min.Analysis of variance (ANOVA) showed that the quadratics of EtOH and extraction temperature had highly significant effect onthe antioxidant capacity (p<0.001). The antioxidant capacity was correlated with contents of bioactive components [crocin,geniposide, and total phenolic (TP) compounds] in gardenia extracts and mainly attributed to the content of the TP compounds.
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Abstract Introduction Materials and Methods Results and Discussion References