Effects of FBS(Feta1 Bovine Serum) and pFF(Porcine Follicular Fluid) on In Vitro Maturation and Development of Porcine Parthenogenetic and Nuclear Transfer Embryos
Hyo-Jin Moon, Joo-Hyun Shim, In-Sun Hwang, Mi-Rung Park, Dong-Hoon Kim, Yeoung-Gyu Ko, Choon-Keun Park, Gi-Sun Im
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https://www.earticle.net/Article/A109575
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ABSTRACT INTRODUCTION MATERIALS AND METHODS Preparation of Porcine Follicular Fluid (pFF) Collection of Oocytes and In Vitro Maturation (IVM) Production of Parthenogenetic (PA) Embryos Brilliant Cresyl Blue Test (BCB) Cdc2 Kinase Assay Apoptosis Assays Preparation of Porcine Fetal Fibroblast Cells Production of Nuclear Transfer (NT) Embryos Statistical Analysis RESULTS Effect of FBS and pFF on In Vitro Maturation and In Vitro Development of Porcine PA and NT Embryos BCB Staining and Cdc2 Kinase Activity of the Oo- cytes Matured in Different Condition DISCUSSION REFERENCES