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Expression of Cyclomaltodextrinase Gene from Bacillus halodurans C-125 and Characterization of Its Multisubstrate Specificity

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 3 (2009.06)바로가기
  • 페이지
    pp.776-781
  • 저자
    Hye-Jeong Kang, Chang-Ku Jeong, Myoung-Uoon Jang, Seung-Ho Choi, Tae-Jip Kim, Min-Hong Kim, Jun-Bae Ahn, Sang-Hwa Lee, Sook-Ja Jo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A108266

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원문정보

초록

영어
A putative cyclomaltodextrinase (BHCD) gene was found from the genome of Bacillus halodurans C-125, whichencodes 578 amino acids with a predicted molecular mass of 67,279 Da. It shares 42-59% of amino acid sequence identity withcommon cyclomaltodextrinase (CDase)-family enzymes. The corresponding gene was cloned by polymerase chain reaction(PCR) and the dimeric enzyme with C-terminal 6-histidines was successfully overproduced and purified from recombinantEscherichia coli. BHCD showed the highest activity against β-CD at pH 7.0 and 50oC. Due to its versatile hydrolysis andtransglycosylation activities, BHCD has been confirmed as a member of CDases. However, BHCD can be distinguished fromother typical CDases on the basis of its novel multisubstrate specificity. While typical CDases have over 10 times higher activityon β-CD than starch or pullulan, the CD-hydrolyzing activity of BHCD is only 2.3 times higher than pullulan. In particular, itshowed significantly higher activity ratio of maltotriose to acarbose than other common CDase-family enzymes.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

Bacillus halodurans C-125 cyclomaltodextrinase (CDase) gene cloning enzymatic characterization multisubstratespecificity

저자

  • Hye-Jeong Kang [ Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea ]
  • Chang-Ku Jeong [ Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea ]
  • Myoung-Uoon Jang [ Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea ]
  • Seung-Ho Choi [ Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea ]
  • Min-Hong Kim [ MH2 Biochemical Co., Ltd., Eumseong, Chungbuk 369-841, Korea ]
  • Jun-Bae Ahn [ Department of Food Service Industry, Seowon University, Cheongju, Chungbuk 361-741, Korea ]
  • Sang-Hwa Lee [ Department of Food and Nutrition, Seowon University, Cheongju, Chungbuk 361-741, Korea ]
  • Sook-Ja Jo [ Department of Food and Nutrition, Seowon University, Cheongju, Chungbuk 361-741, Korea ]
  • Tae-Jip Kim [ Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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