Effect of High Speed Drying on Antioxidant Properties of Enzymatic Digests from Citrus By-products and Their Protective Effect on DNA Damage Induced by H2O2
Mahinda Senevirathne, Soo-Hyun Kim, Jin-Hwan Ha, Byung-Hun Um, Jung-Suk Lee, Won-Woo Lee, You-Jin Jeon
언어
영어(ENG)
URL
https://www.earticle.net/Article/A108242
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원문정보
초록
영어
The aim of this study was to evaluate the antioxidant activities of enzymatic digests from citrus by-products(CBPs) prepared by high speed drying (HSD). HSD needs a short time (60 min) for drying and can be used in a commercialscale. Enzymatic digests were prepared from the CBPs using 6 enzymes such as aminoglucosidase (AMG), celluclast,pectinex, termamyl, ultraflo, and viscozyme. Antioxidant activities of AMG digest from CBPs were evaluated by different invitro models such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, alkyl, H2O2 scavenging, metal chelating, lipid peroxidation,and comet assays, and exhibited strong activities. The antioxidant compounds were detected by an high performance liquidchromatography (HPLC) coupled on-line to an 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) scavengingdetection system, and found that hesperidin was the key compound. Therefore, the results obtained in this study suggest thatHSD is an effective method to transform wet CBPs into dried form, and CBPs are potential source of natural antioxidant.
목차
Abstract Introduction Materials and Methods Results and Discussion References
키워드
antioxidant activitycitrus by-productenzymatic digesthigh speed dryingHPLC coupled on-line to an ABTS+
저자
Mahinda Senevirathne [ Department of Food Bioengineering, Jeju National University, Jeju, Jeju 690-756, Korea ]
Soo-Hyun Kim [ Department of Food Bioengineering, Jeju National University, Jeju, Jeju 690-756, Korea ]
Byung-Hun Um [ Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Gangwon 210-340, Korea ]
Jung-Suk Lee [ Aqua Green Tech Co., Jeju, Jeju 690-150, Korea ]
Jin-Hwan Ha [ Department of Food Bioengineering, Jeju National University, Jeju, Jeju 690-756, Korea ]
Won-Woo Lee [ School of Marine Biomedicinal Science, Jeju National University, Jeju, Jeju 690-756, Korea ]
You-Jin Jeon [ School of Marine Biomedicinal Science, Jeju National University, Jeju, Jeju 690-756, Korea ]
Corresponding author