Won-Bo Shim, Gyeongyeol Kim, Hee-Jung Ryu, Minji Nam, Duck-Hwa Chung
언어
영어(ENG)
URL
https://www.earticle.net/Article/A108235
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원문정보
초록
영어
A one-step simultaneous immunchromatographic (OS-ICG) assay using colloidal gold-monoclonal antibody(gold-MAb) conjugates was developed for the rapid multianalysis of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in feedsamples. Visual detection limits for AFB1 and OTA were 0.5 and 2.5ng/mL, respectively, and the results were obtainedwithin 15min. Matrix interference from the feed extracts was efficiently reduced by appropriate dilution with buffer. Cut-offvalues of the OS-ICG assay for the feed spiked with AFB1/OTA mixtures (5/5, 10/10, 25/25, 50/55, 100/100µg/kg) were 10and 50µg/kg for AFB1 and OTA. The comparative analyses of 65 feed samples by OS-ICG, enzyme-linked immunosorbentassay (ELISA), and high performance liquid chromatography (HPLC) showed good agreement. In this study, we confirmedthat simultaneous analysis based on immunoassay is possible and it can be used as an on-site multianalysis of AFB1 and OTAin feed, food, and agricultural products.
목차
Abstract Introduction Materials and Methods Results and Discussion References
Won-Bo Shim [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Gyeongyeol Kim [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Hee-Jung Ryu [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Minji Nam [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Duck-Hwa Chung [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
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