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Development of One-step Simultaneous Immunochromatographic Assay for Rapid Analysis of Aflatoxin B1 and Ochratoxin A

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 3 (2009.06)바로가기
  • 페이지
    pp.641-648
  • 저자
    Won-Bo Shim, Gyeongyeol Kim, Hee-Jung Ryu, Minji Nam, Duck-Hwa Chung
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A108235

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원문정보

초록

영어
A one-step simultaneous immunchromatographic (OS-ICG) assay using colloidal gold-monoclonal antibody(gold-MAb) conjugates was developed for the rapid multianalysis of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in feedsamples. Visual detection limits for AFB1 and OTA were 0.5 and 2.5ng/mL, respectively, and the results were obtainedwithin 15min. Matrix interference from the feed extracts was efficiently reduced by appropriate dilution with buffer. Cut-offvalues of the OS-ICG assay for the feed spiked with AFB1/OTA mixtures (5/5, 10/10, 25/25, 50/55, 100/100µg/kg) were 10and 50µg/kg for AFB1 and OTA. The comparative analyses of 65 feed samples by OS-ICG, enzyme-linked immunosorbentassay (ELISA), and high performance liquid chromatography (HPLC) showed good agreement. In this study, we confirmedthat simultaneous analysis based on immunoassay is possible and it can be used as an on-site multianalysis of AFB1 and OTAin feed, food, and agricultural products.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

multianalysis aflatoxin B1 ochratoxin A one-step immunochromatographic assay

저자

  • Won-Bo Shim [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Gyeongyeol Kim [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Hee-Jung Ryu [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Minji Nam [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Duck-Hwa Chung [ Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 18 Number 3

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