Earticle

초록

영어

Mitochondrial DNA (mtDNA) deletion is a well-known marker for oxidative stress and aging and also contributes to theirunfavorable effects in cultured cells and animal tissues. This study was conducted to investigate the effect of ionizingradiation (IR) on mtDNA deletion and the involvement of reactive oxygen species (ROS) in this process in human lungfibroblast (IMR-90) cells. Young IMR-90 cells at population doubling (PD) 39 were irradiated with 137Cs -rays and theintracellular ROS level was determined by 2',7'-dichlorofluorescein diacetate (DCFH-DA) and mtDNA common deletion(4977bp) was detected by nested PCR. Old cells at PD 55 and H2O2-treated young cells were compared as the positivecontrol. IR increased the intracellular ROS level and mtDNA 4977 bp deletion in IMR-90 cells dose-dependently.Theincreases of ROS level and mtDNA deletion were also observed in old cells and H2O2-treated young cells. To confirmtheincreased ROS level is essential for mtDNA deletion in irradiated cells, the effects of N-acetylcysteine (NAC) on IR-induced ROS and mtDNA deletion were examined. 5 mM NAC significantly attenuated the IR-induced ROS increaseand mtDNA deletion. These results suggest that IR induces the mtDNA deletion and this process is mediated by ROS inIMR-90 cells.

목차

abstract
 1. INTRODUCTION
 2. MATERIALS AND METHODS
 3. RESULTS AND DISCUSSION
 4. CONCLUSION
 REFERENCES

키워드

저자

• HYEON SOO EOM [ Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute ]
• UHEE JUNG [ Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute ]
• HAE-RAN PARK [ Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute ]
• SUNG-KEE JO [ Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  방사선방어학회지 [Journal of Radiation Protection and Research]

• 간기

  계간

• pISSN

  2508-1888

• 수록기간

  1976~2026

• 등재여부

  KCI 등재,SCOPUS

• 십진분류

  KDC 559 DDC 629