Earticle

초록

영어

Placenta is the main nutrition source for the fetus during pregnancy. Thus, it has a pivotal function in the pregnant process. Many functions of the placenta have been elucidated. An abnormal placenta is associated with a high rate of pregnancy failure in somatic cloned bovine. Differentially expressed genes (DEGs) were examined in a comparison between normal and cloned bovine placenta using annealing control primer (ACP)-based GeneFishing PCR. Using 120 ACPs, nearly 80 genes were identified and the fragments of 42 DEGs were sequenced. 38 of these genes were known genes and four were unknown. To determine the DEGs result, six target clones expressing on one-side of a normal and a clone placenta were selected. Through an analysis of the target genes using the real-time PCR, the expressing pattern was found to be somewhat different from the DEGs. Additionally, several genes appeared with the same expression pattern. Taken together, this suggests that the target genes would be essential for research into what influences the placental formative mechanisms during fetal development.

목차

ABSTRACT
 INTRODUCTION
 MAERIALS AND METHODS
  Source for Nuclear Transferred Somatic Cells and Nuclear Transfer
  Generation of Placental Tissues
  RNA Isolation
  cDNA Synthesis and ACP RT-PCR Analysis
  Cloning and Sequencing
  Semi-Quantitative RT-PCR
  Real-Time PCR
  Statistical Analysis
 RESULTS
  Analysis of DEGs in Cloned Bovine Placenta
  Semi-Quantitative RT-PCR
  Real-Time PCR
 DISCUSSION
 REFERENCES

키워드

저자

• Hee-Ja Park [ Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Yeoung-Gye Ko [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Byoung-Chul Yang [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Hwan-Hoo Seong [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Seok-Doo Oh [ Department of Animal Science & Biotechnology, Jinju National University ]
• Sue-Yun Hwang [ Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Kwan-Sik Min [ Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA ]
• Jong-Taek Yoon [ Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636