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Enzymatic Preparation of Maltooctaose-rich Mixture from Starch Using a Debranching Enzyme of Nostoc punctiforme

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 2 (2009.04)바로가기
  • 페이지
    pp.570-573
  • 저자
    Ji-Hye Choi, Jong-Tae Park, Kwan-Hwa Park, Myo-Jeong Kim, Young-Wan Kim, Heeseob Lee, Byong-Hoon Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A105516

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원문정보

초록

영어
The debranching enzyme of Nostoc punctiforme (NPDE) is a novel enzyme that catalyzes the hydrolysis of α-1,6-glycosidic linkages in starch, followed by the sequential hydrolysis of α-1,4-glycosidic linkages. The debranching activity ofNPDE is highly specific for branched chains with a degree of polymerization (DP)>8. Moreover, the rate of hydrolysis of α-1,4-linkages by NPDE is greatly enhanced for maltooligosaccharides (MOs) with a DP>8. An analysis of reaction mixturescontaining various starches revealed the accumulation of maltooctaose (G8) with glucose and maltose. Based on the novelenzymatic properties of NPDE, an MO mixture containing more than 60% G8 with yield of 18 g G8 for 100g starch wasprepared by the reaction of NPDE with soluble starch, followed by ethanol precipitation and gel permeation chromatography(GPC). The yield of the G8-rich mixture was significantly improved by the addition of isoamylase. In summary, a 4-stepprocess for the production of a G8-rich mixture was developed involving the enzymatic hydrolysis of starch by NPDE.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

Nostoc punctiforme cyanobacteria debranching enzyme maltooctaose-rich mixture maltooligosaccharide

저자

  • Ji-Hye Choi [ Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921, Korea ]
  • Myo-Jeong Kim [ Food Research Institute and School of Food and Life Science, and Biohealth Products Research Center, Inje University, Gimhae, Gyeongnam 621-749, Korea ]
  • Young-Wan Kim [ Department of Food and Biotechnology, Korea University, Chochiwon, Chungnam 339-700, Korea ]
  • Heeseob Lee [ Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Korea ]
  • Jong-Tae Park [ Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921, Korea ]
  • Byong-Hoon Lee [ Department of Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4/AAFC, Canada ]
  • Kwan-Hwa Park [ Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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