Hee-Do Hong, Eun Mi Sim, Kyungtack Kim, Jeonghae Rho, Young Kyung Rhee, Chang-Won Cho
언어
영어(ENG)
URL
https://www.earticle.net/Article/A105515
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원문정보
초록
영어
This study was conducted to evaluate the effects of different preparation methods on the recovery and quantificationof ginsenosides in raw Korean ginseng (Panax ginseng C.A. Meyer). Eight major ginsenosides (Rb1, Rb2, Rb3, Rc, Rd, Re, Rf,and Rg1) were analyzed by high performance liquid chromatography (HPLC), after which the recovery and repeatability of theextraction of those ginsenosides using 3 different preparation methods were compared [A. direct extraction (DE) method, hotMeOH extraction/evaporation/direct dissolution; B. solid phase extraction (SPE) method, hot MeOH extraction/evaporation/dissolution/C18 cartridge adsorption/MeOH elution; C. liquid-liquid extraction (LLE) method, hot MeOH extraction/evaporation/dissolution/n-BuOH fractionation]. Use of the DE method resulted in a significantly higher recovery of totalginsenosides than other methods and a relatively clear peak resolution. Use of the SPE and LLE methods resulted in clearerpeak resolution, but lower ginsenoside recovery than the DE method. The LLE method showed the lowest ginsenosiderecovery and repeatability among the 3 methods. Given that the DE method employed only extraction, evaporation, and adissolution step (avoiding complicate and time consuming purification), this technique may be an effective method for thepreparation and quantification of ginsenosides from raw Korean ginseng.
목차
Abstract Introduction Materials and Methods Results and Discussion References
키워드
Korean ginsengginsenoside analysisextractionpurificationhigh performance liquid chromatography
저자
Hee-Do Hong [ Regional Food Industry Research Group, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea ]
Eun Mi Sim [ Regional Food Industry Research Group, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea ]
Kyungtack Kim [ Regional Food Industry Research Group, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea ]
Jeonghae Rho [ Regional Food Industry Research Group, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea ]
Young Kyung Rhee [ Regional Food Industry Research Group, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea ]
Chang-Won Cho [ Regional Food Industry Research Group, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea ]
Corresponding author