Earticle

초록

영어

A bacterial strain (Strain N-08) capable of extracellularly producing high level of non-reducing oligosaccharide(NR-OS) isolated from soil. The strain was identified phylogenetically by 16S rDNA sequence analysis and found to be veryclose to Arthrobacter crystallopoietes. The high production of NR-OS was observed in the basal culture medium containingmaltose as a sole carbon source. The NR-OS in culture supernatant was purified by glucoamylase treatment and Dowex-1(OH−) ion exchange chromatography and its structure was characterized. This oligosaccharide consisted of only glucose.Methylation analysis indicated that this fraction was composed mainly of non-reducing terminal glucopyranoside. Matrix-assisted laser-induced/ionization time-of-flight (MALDI-TOF) and electrospray ionization-mass spectrometry (ESI-MS)/MSanalysessuggested that this oligosaccharide comprised non-reducing disaccharide unit with 1,1-glucosidic linkage. When thisdisaccharide was analyzed by 1H-NMR and 13C-NMR, it gave the same signals with α-D-glucopyranosyl-(1,1)-α-D-glucopyranoside. These results indicated that the NR-OS produced by A. crystallopoietes N-08 was α1,α1-trehalose. This isthe first report of the trehalose which can be produced directly from maltose by A. crystallopoietes N-08.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

저자

• Bum Sun Bae [ Department of Food Science and Biotechnology, Kyonggi University, Suwon, Gyeonggi 443-760, Korea ]
• Kwang-Soon Shin [ Department of Food Science and Biotechnology, Kyonggi University, Suwon, Gyeonggi 443-760, Korea ]
• Ho Lee [ Department of Food Science and Biotechnology, Kyonggi University, Suwon, Gyeonggi 443-760, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664