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Statistical Optimization of the Medium Components for the Production of Protopectinases by Bacillus subtilis

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 2 (2009.04)바로가기
  • 페이지
    pp.442-448
  • 저자
    Nafise Shahbazian, Farzin Zokaee Ashtiani, Babak Bonakdarpour
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A105494

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원문정보

초록

영어
In this study Bacillus subtilis PTCC 1023 was used for the production of protopectinase using soybean basedmedia. The use of isolated soybean protein (ISP) and soybean flour resulted in similar protopectinase production and growthrates. The effect of medium composition on protopectinase production was studied using central composite design (CCD)methodology. The change in the concentration of ISP (1-7%), glucose (0-10%), and phosphate (0.1-0.3 M) was found to affectthe protopectinase activity (response variable) after 24 hr of cultivation. In the range studied, ISP and glucose had a negativeeffect on the response variable, whereas phosphate had a positive effect. A statistically significant interaction was identifiedbetween phosphate and ISP, suggesting that correct optimization of medium formulation in this case can only be obtainedusing factorial design of experiments. Protopectinase activity exceeding 215 U/mL was obtained in a medium containing 4%ISP, 0.3M phosphate, and no added sugar.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

pectinolytic enzyme protopectinase Bacillus subtilis isolated soybean protein central composite design

저자

  • Nafise Shahbazian [ Department of Chemical Engineering, Amirkabir University of Technology, Hafez Avenue, Tehran, Iran ]
  • Farzin Zokaee Ashtiani [ Department of Chemical Engineering, Amirkabir University of Technology, Hafez Avenue, Tehran, Iran; Food Process Engineering and Biotechnology Research Centre, Amirkabir University of Technology, Hafez Avenue, Tehran, Iran ] Corresponding author
  • Babak Bonakdarpour [ Department of Chemical Engineering, Amirkabir University of Technology, Hafez Avenue, Tehran, Iran; Food Process Engineering and Biotechnology Research Centre, Amirkabir University of Technology, Hafez Avenue, Tehran, Iran ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 18 Number 2

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