Earticle

초록

영어

The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to atranscriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putativecountertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, forvarious lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistancegene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis,Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traitsof transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E.coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

저자

• Do-Won Jeong [ Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea ]
• San Ho Cho [ Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea ]
• Jong-Hoon Lee [ Department of Food Science and Biotechnology, Kyonggi University, Suwon 443-760, Korea ]
• Hyong Joo Lee [ Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664