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Inactivation Mechanism of Bacillus subtilis Spores by Ethanol Extract of Torilis japonica Fruit

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 2 (2009.04)바로가기
  • 페이지
    pp.336-342
  • 저자
    Won-Il Cho, Jun-Bong Choi, Kangpyo Lee, Chan-Ick Cheigh, You-Jung Choi, Jeong-Yoon Jeong, Myong-Soo Chung, Seok Cheol Cho, Yu-Ryang Pyun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A105476

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원문정보

초록

영어
To confirm the antimicobial mechanism of Torilis japonica, antimicrobial profile was observed on various sporeconditions by combining 0.1% (3 mM) torilin with antimicrobial activity and 0.27% water fraction with germinants. A 75%ethanol extract of T. japonica fruit reduced Bacillus subtilis ATCC 6633 spore counts by 3 log cycles and reduced thevegetative cells to undetectable level (by about 6 log cycles) (both in terms of CFU/mL). Further fractionating the ethanolextract into n-hexane and water fractions revealed that the former reduced the spore count by 1 log cycle whereas the latterhad no effect. The antimicrobial active compound was isolated and purified from the hexane layer, and identified as torilin(C22H32O5). The water fraction of the ethanol layer did not show antimicrobial activity, whereas the antimicrobial effect of0.1% (3mM) torilin was significantly enhanced in the presence of the water fraction (0.27%). This result can be explained bysynergistic effects of the water fraction containing considerable amounts of germinants such as L-alanine and K+ ions thattriggered germination.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

Bacillus subtilis endospore Torilis japonica fruit torilin germinant

저자

  • Won-Il Cho [ CJ Foods R&D, CJ Corp., Seoul 152-050, Korea ]
  • Chan-Ick Cheigh [ Department of Food Science and Engineering, Ewha Womans University, Seoul 120-750, Korea ]
  • You-Jung Choi [ Department of Food Science and Engineering, Ewha Womans University, Seoul 120-750, Korea ]
  • Jeong-Yoon Jeong [ Department of Food Science and Engineering, Ewha Womans University, Seoul 120-750, Korea ]
  • Jun-Bong Choi [ CJ Foods R&D, CJ Corp., Seoul 152-050, Korea ]
  • Kangpyo Lee [ CJ Foods R&D, CJ Corp., Seoul 152-050, Korea ]
  • Seok Cheol Cho [ Bio Van Co., Ltd., Yonsei University, Seoul 120-749, Korea ]
  • Yu-Ryang Pyun [ Bio Van Co., Ltd., Yonsei University, Seoul 120-749, Korea ]
  • Myong-Soo Chung [ Department of Food Science and Engineering, Ewha Womans University, Seoul 120-750, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 18 Number 2

    피인용수 : 0(자료제공 : 네이버학술정보)

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