Jeong-Ok Lee, Eun-Jung Kim, Yu-Jin Ko, Chung-Ho Ryu, Sang-Il Lee, Won-Sup Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A105473
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원문정보
초록
영어
The proteins from 15 types of cultivar of hot peppers cultivated in Korea were extracted and its allergenicity wasinvestigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA). The immunoblotting of hot pepperproteins extracts (HPEs) against serum of hot pepper sensitized patients revealed dominant IgE binding to 14, 37, and 40 kDamolecules. The specific levels of IgE to HPEs sample No. 1, 3, and 7 were much higher than the other samples in patients.Also, IgE binding capacity of HPEs were not reduced by thermal processing and digestion in ELISA using human IgEantibody acquired from hot pepper sensitized patients. By means of Western blotting using anti-thaumatin IgY, thaumatin-likeprotein (TLP) acting as allergen in several plants and fruits was detected in tested hot peppers. This study demonstrates thatthe antigenic protein in hot peppers are present but are differently contained according to cultivars.
목차
Abstract Introduction Materials and Methods Results and Discussion References
키워드
hot pepperCapsicum annuumfood allergythaumatin-like proteinallergenicity
저자
Jeong-Ok Lee [ Division of Applied Life Science (BK 21 Program) and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Eun-Jung Kim [ Division of Applied Life Science (BK 21 Program) and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Yu-Jin Ko [ Division of Applied Life Science (BK 21 Program) and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Sang-Il Lee [ Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea ]
Won-Sup Lee [ Department of Internal Medicine, Gyeongsang National University Hospital, Jinju, Gyeongnam 660-702, Korea ]
Chung-Ho Ryu [ Division of Applied Life Science (BK 21 Program) and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Corresponding author