Earticle

초록

영어

Antioxidative and matrix metalloproteinase-2 and -9 (MMP-2 and -9) inhibitory effects of carboxymethyl-chitosan and -chitin (CM-chitosan and -chitin) were investigated in HT1080 cells. CM-chitosan and CM-chitin suppressed intracellular ROS formation by DCFH-DA and radical simulated oxidations of membrane protein and lipid in a concentrationdependent
manner. In addition, a protective effect against oxidative damage of purified genomic DNA was observed in presence of CMchitosan and CM-chitin. Moreover, CM-chitosan and CM-chitin reduced the expression levels of MMP-2 and -9 in gelatin zymography, RT-PCR and western blot analysis without any cytotoxic influence. CM-chitin exhibited a higher MMP inhibitory effect than CM-chitosan through transcriptional down-regulations of AP-1 and NF-κB. Findings from the
present study should underscore the nutraceutical value of CM-chitosan and CM-chitin as a potent anti-oxidant and MMP inhibitor via alleviations of radical-induced oxidative damage.

키워드

저자

• Jung-Ae KIM [ Department of Chemistry, Pukyong National University ]
• Chang-Suk KONG [ Marine Bioprocess Research Center, Pukyong National University ]
• Byulnim AHN [ Department of Chemistry, Pukyong National University ]
• Tae-Kil EOM [ Department of Chemistry, Pukyong National University ]
• Se-Kwon KIM [ Department of Chemistry, Pukyong National University ]
• Hee-Guk BYUN [ Faculty of Science and Biotechnology, Kangnung National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576