Earticle

초록

영어

Variable glycosylation of antibodies occurs at Asn297 in the Fc region, specifically terminal galactosylation and sialylation. These variations may affect both pharmacokinetic behavior and effecter function, such as complement-dependant cytotoxicity (CDC).In this study, glucose, as a
carbon source in culture medium, was substituted for the other sugars in an attempt to induce greater in vivo efficacy of recombinant antibodies and increase their potential as therapeutic drugs by altering glycosylation of antibody. Suspension cultures of recombinant CHO cells were grown in protein-free media, and purification and analysis were performed using liquid chromatographic methods. The results demonstrated that glucose and mannose promote cell growth and lactate production, while galactose and fructose were found to be good energy sources for glycosylation. So, it was proven that the more lactate was produced, the less glycosylation of recombinant antibody was progressed. However, there was a synergistic
effect on glycosylation when mannose was used with other sugars.In summary, carbon sources in culture medium can affect glycosylation of recombinant antibodies. These results provide a reference point for the development of therapeutic recombinant antibodies in the industry.

키워드

저자

• Seong Min KIM [ Department of Bioscience&Biotechnology, Sejong university ]
• Duk Jae OH [ Department of Bioscience&Biotechnology, Sejong university ]
• Jang Hyeon PARK [ BioR&D Co., Ltd. 506 ChungMu-Bldg., 98 Gunja-dong, Gwangjin-Gu, Seoul 143-747. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576