Earticle

초록

영어

Encapsulation of functional proteins inside hydrophilic core space of vesicular liposomes is an important issue in such applications of targeted delivery of therapeutic proteins, immobilized reactions of industrial enzymes [1], and bionano sensing of diagnostic proteins. The encapsulation yield is generally quite low and needs to be improved for the applications to be industrially viable. Several parameters are known to affect the yield [2]; liposomal lipids
types, liposome size, buffer type, pH and ionic strength, protein size (or, molecular weight). Enzymes were selected as an encapsulant because it can replace protein drug due to physical and chemical similarity and enable to measure bioactivity. In this study, by fixing the liposomal lipid as DPPC (dipalmitoylphosphatidylcholine) and using various sized proteins as model
proteins (i.e., trypsin, 15 kDa; horseradish peroxidase, 44 kDa; hyaluronidase, 80 kDa; enterokinase, 250 kDa), we evaluated the effects of these parameters on encapsulation yield. After encapsulation process, size exclusion chromatography (Sepharose 4B) was applied to separate liposomes and free proteins [3]. Encapsulation yield was calculated in two units; volumetric encapsulation (mole of protein per unit volume) and surface encapsulation (mole of protein per unit surface area). Effects of buffer pH and salt concentration on proteinlipid
interaction were measured by surface plasmon resonance. 5 mM of DPPC liposome dissolved 6 ml of 1mg/ml of enzyme solution represented over 50% of encapsulation yield. These encapsulation properties demonstrated optimal condition to achieve high encapsulation yield with more than 80% of initial bioactivity.

키워드

저자

• Hak Kyong KIM [ Dept. of Chemical Engineering, Hanyang uninversity, Ansan. ]
• Eun Kyu LEE [ Dept. of Chemical Engineering, Hanyang uninversity, Ansan. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576