Earticle

초록

영어

Electrochemical concentration and enumeration of bacteria using an amperometric sensor system were investigated. Electrochemical reduction and oxidation reaction of bacteria in the presence of an electrochemical mediator are basis of the sensor system. When a charged electrode (graphite felt, +0.68V) was incorporated in a bacterial culture broth with a mediator (MB 0.01 mM), about 20% decrease in bacterial number was observed. The bacteria attached
electrode was then used as a working electrode for a three-electrode electrochemical system for the bacteria enumeration. In the system, the amperometric reoxidation current and its rate of increment were found to be dependent on the concentrations of bacteria and mediator. Linear
correlations between initial rate currents and bacterial concentrations were established. Methylene Blue (MB) was selected as a most appropriate redox mediator, and the system was most sensitive and stable using a working electrode poised at 0.3 V (vs. the reference), pH
7.0, an MB concentration of 0.0001 M, a working electrode surface area of 550 mm2, and a temperature of 30 ℃.

키워드

저자

• Beom-Yong LEE [ Dept. of Microbial Engeneering, Konkuk University, Seoul. ]
• Kyu-Won SEO [ Dept. of Microbial Engeneering, Konkuk University, Seoul. ]
• Seong-Min LEE [ Dept. of Microbial Engeneering, Konkuk University, Seoul. ]
• Jeong-Soo PARK [ Dept. of Microbial Engeneering, Konkuk University, Seoul. ]
• Moon-Sik HYUN [ Dept. of Microbial Engeneering, Konkuk University, Seoul. ]
• Hyung-Joo KIM [ Dept. of Microbial Engeneering, Konkuk University, Seoul. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576