Nanosurface consisting of nano structures was developed to enhance the surface density of capture protein on spot in protein chip array. Silica nano pillar was formed from nanoporous alumina template using sol-gel method. Silica nano pillars were transferred to PDMS layer prior to remove the alumina template. Silica nano structure was achieved with individual pillars by their aggregations because they have high aspect ratio (length: 1000 nm, diameter: 80 nm). Nano structure has 600 nm of height and 1000 nm of diameter. The rose’s petal effect was achieved with nanosurface because nanostructure contains individual nano pillars. Nanosurface consisting of nanostructures was investigated using AFM(Atomic Force Microscopy) and SEM (Scanning Electron Microscopy). The amount of protein immobilized on nanosurface was compared with that on normal flat glass. Nanosurface developed in this study can be applied to nanoscaled biochip. Acknowledgments: This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10755160002-07N5516-00210).
키워드
NanostructureProtein arraySilica
저자
So Yeon KIM [ Colleage of Bionanotechnology, Kyungwon University ]
Sang Jun SON [ Colleage of Bionanotechnology, Kyungwon University ]
Junhong MIN [ Colleage of Bionanotechnology, Kyungwon University ]
Jeaeun YU [ Department of Chemistry, Seoul National University, Seoul 151-747, Repubilic of Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동