Tae Jung PARK, Do-Kyun KIM, Heeyon KIM, Sang Yup LE E
언어
영어(ENG)
URL
https://www.earticle.net/Article/A105373
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원문정보
초록
영어
Aflatoxins are a group of metabolites produced by Aspergillus flavus and Aspergillus parisiticus, which are found in a variety of agriculture products. Many sensitive methods for the analysis of Aflatoxins in food and feed, such as thin-layer chromatography, high-performance liquid chromatography, enzyme-linked immunosorbant assay and so on, have developed. Whereas these methods are offering good detection limits, they are time consuming and need expensive instruments. Thus, the development of a rapid and convenient detection biosensor for Aflatoxin analysis is extremely desirable. In order to investigate the biosensor for the food safety, we performed optical resonance methods which are simple, rapid and sensitive methods on the multi-spot nanoparticle array chip surface. The multi-spot nanoparticle array chip was applied to the localized surface plasmon resonance (LSPR)-based optical biosensor for monitoring the protein antibodyantigen interaction. For the detection of Aflatoxin, we used the specific binding between antibody and antigen, and synthesized gold binding polypeptide-Protein G fusion protein having the specific binding affinities against the multi-spot nanoparticle array chip surface and immunoglobulin G, respectively. In this study, we successfully developed the label-free LSPR-based biosensor whose the detection limit of 100 pg/mL by using the anti-Aflatoxin B1 antibody.
Tae Jung PARK [ BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
Do-Kyun KIM [ BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
Heeyon KIM [ Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701. ]
Sang Yup LE E [ Department of Chemical & Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, Institute for the BioCentury, Department of Bio & Brain Engineering, Department of Biological Sciences, and BioInformatics Research Center, KAIST, Daejeon 305-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동