Earticle

초록

영어

Cell microarrays could constitute new weapons of mass analysis in the arsenal of biologists and pharmacists. The advantage of cell microarrays is the opportunity to measure parameters on hundreds of individual cells and average them, instead of measuring the parameters of a whole cell population as well as the increased sensitivity as assay volume shrinks. “Shape encoded cell array” does not rely on x and y coordinates on an array to identify the cell type compared with ‘Positional cell array’, but on a ‘shape code.’ In this study, we fabricated various shapes of PEG hydrogel microparticles.. PEG hydrogel microparticles were photocrosslinked with a small amount of acrylic acid to provide functional groups that enables protein immobilization via EDC/NHS method in the PEGDA(poly(ethyleneglycol) diacrylate, Mw700) precursor. After that,
NIH-3T3 cell, a species of fibroblast, or hepatocyte G2 were seeded on the surface of the hydrogel microparticles with different shapes and sizes. We obtained cell viability ratio with fluorescent live/dead assay, and monitored cell density on the surface of each hydrogel microparticle. The advantages of this technique are facile recognition of different type
of cells by the shape of the hydrogel particles, and the process of substrate preparation that is fabricated by a single step of photopatterning.

키워드

저자

• Saemi PARK [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]
• Yeol LEE [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]
• Dae Nyun KIM [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]
• Sangphil PARK [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]
• Eunji JANG [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]
• Hyun Jong LEE [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]
• Seung Hee NAM [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]
• Won-gun KOH [ Department of Chemical and Biomolecular Engineering, Yonsei University. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576