Earticle

초록

영어

It’s the most important to provide environment similar with in vivo, to obtain satisfactory
results in vitro experiment. Thus the importance of 3-D cell culture is emphasized recently in vitro cell culturing experiment. Also, the scaffold architecture is very important and affects cell binding.To observe specific sites or cells are difficult in a normal cell culture system. The alternative to solve the problem in the normal cell culture is surface patterning. Surface patterning is routinely used to immobilize bioactive molecules such as proteins, oligonucleotides and small ligands, to localize surface reactions for bioassays and to provide desired cell and bacterial adhesion.In this study, we proposed a new 3-D cell culture and cell assay combined system. We mimic extracellular matrix (ECM) via electrospinning. Electrospinning generates loosely
connected 3D porous mats with high porosity and high surface area. PEG hydrogel was
patterned through photolithography on the electrospun fiber scaffold.Morphology of fabricated hydrogel patterned 3-D scaffold was observed using confocal and SEM microscopes. The fluorescence images of the shapes and mask effect of hydrogel patterns were observed via TRITC-dextran and FITC-BSA adsorption experiment.We were able to corroborate the interaction between cells and fabricated scaffolds through SEM, inverted microscope and viability of cells.So, we expect this new scaffold and cell culture system will be applied for biotechnological culture supports, implanted biosensors, neural interfaces, and other structural medical implants engineered with nanoscale features to reduce the likelihood of fibrous encapsulation and allow stronger interfacing with the host tissue for a longer period of time.

키워드

저자

• Hyun Jong LEE [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]
• Yeol LEE [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]
• Dae Nyun KIM [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]
• Sang Phil PARK [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]
• Saemi PARK [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]
• Eunji JANG [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]
• Seung Hee NAM [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]
• Won-Gun KOH [ Dept. of Chemical and Biomolecular Engineering, Yonsei University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576