Cheol-Heon YEA, Jeong-Woo CHOI, Md. Abdul KAFI, Waleed Ahmed EL-SAID
언어
영어(ENG)
URL
https://www.earticle.net/Article/A105364
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Cell chip is becoming a popular tool for monitoring and detecting a drug or other effects. This system was used for monitoring the neurotransmitter chemicals (dopamine) released from a neural cell (PC12). Upon the cells grow on a chip, dopamine can be detected by three-electrode cyclic voltammetry (CV) method. A cyclic voltammogram of PC12 cells grown on collagen coated gold showed that current peaks were linearly increased with increase of the cell number. The anodic and cathodic peaks are the same potential range for both cellular and chemical dopamine, and it is confirmed that potassium and glucose have a stimulatory effect on dopamine production by the cell. Current peak increased with increase of potassium and glucose concentrations, and the increase was the greatest when potassium and glucose are used together. The sensitivity of detecting can be increased using potassium and glucose stimulation. These results indicate that CV is an effective method for monitoring of dopamine production from PC12 cells. Using other cell lines and evaluation of drug effect (toxic agent) on this cell chip is on going. Acknowledgments: This research was supported by The Nano/Bio Science & Technology Program (M10536090001-05N3609-00110) of the Ministry of Education, Science and Technology (MEST), Ministry of Environment of the Republic of Korea as "The Eco-technopia 21 project" and by Seoul R&BD Program (10816).
키워드
NanobiochipNeural cell chipNeurotransmitter
저자
Cheol-Heon YEA [ Dept. of Chemical & Biomolecular Engineering, Sogang University ]
Jeong-Woo CHOI [ Dept. of Chemical & Biomolecular Engineering, Sogang University ]
Md. Abdul KAFI [ Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 121-742. ]
Waleed Ahmed EL-SAID [ Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 121-742. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동