Earticle

초록

영어

An ion sensitive field effect transistor (ISFET) as a transducer, more precisely immunologically modified FET (immunoFET), was used to detect C-reactive protein (CRP). The investigated ISFET biosensor was fabricated by utilizing a standard CMOS process. Anti-CRP monoclonal antibody was attached on the gate surface using protein G as a linker protein for orientation-controlled immobilization. The response of CRP antibody to CRP was evaluated by measuring the
electrical features of the ISFET device. As a result, a considerable decrease in drain current was clearly observed upon CRP binding to anti-CRP antibody on the gate, which can be explained by the charge effect that negatively charged CRP is responsible for the current drop. Collectively, our data convincingly indicated that FET-type biosensor is potentially applicable for the detection of CRP through the antigenantibody binding event on a gate surface.

키워드

저자

• Moonil KIM [ BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB). ]
• Hye-Jung PARK [ BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB). ]
• Bong Hyun CHUNG [ School of Engineering, University of Science and Technology (UST). ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576