Earticle

초록

영어

T lymphocyte adhesion to adhesion molecules on endothelium is important in immune function, cancer metastasis and inflammation. This cell-cell binding is controlled via cell adhesion molecules such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) of endothelial cells and adhesive properties of T-cell which is greatly modified in several diseased conditions. Thus, therapeutic agents that down-regulate leukocyte-endothelial interactions have been focused to markedly regulate the progression of inflammatory responses in a number of in vivo models. Here, we present a protein based microfluidic system that mimics an inflammatory site which occur the binding of leucocytes to endothelium. This system can easily monitor the binding leucocytes to adhesion molecules under various physiological conditions, such as, shear stress and the density of adhesion molecules. We fabricate protein based microfluidic system by immobilization of cell adhesion molecules (ICAM-1, VCAM-1, E-selectin) on the glass surface. The T cell binding to cell adhesion molecules were affected by the height of channel, density of adhesion molecules and shear stress. The maximum binding was observed at 2 dyne/cm2 of shear stress and 20 ug/ml of the density of adhesion
molecule in the case of ICAM-1. This system is easily integrated with SPR as detection method to monitor the dynamics of reactions in real time. Furthermore, this device is useful as a biosensor for clinical diagnostics and high throughput drug screening.

키워드

저자

• Won Kang MOON [ Dept. of Biotechnology, Yonsei University, Seoul, 120-749. ]
• Min Kyeong SHIN [ Dept. of Biotechnology, Yonsei University, Seoul, 120-749. ]
• Huisuk YANG [ Dept. of Biotechnology, Yonsei University, Seoul, 120-749. ]
• Hyungil JUNG [ Dept. of Biotechnology, Yonsei University, Seoul, 120-749. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576