Microarrays can be used to screen thousands of binding events in a parallel and high throughput fashion, and are of major importance in the detection of a disease and drug discovery. The use of radioisotope (RI) is conventionally regarded as one of the most sensitive detection methods. However, it takes a long time to implement a data analysis for a signal intensity. A glass chip is exposed to an X-ray film or imaging plate and then developed or analyzed by a bioimage analyzer. Radioactive labeling is mainly performed using different RI’s such as 33P and 32P into ATP for a phosphorylation. We reported here on the degree of incorporation of 33P and 32P into a substrate measured by a radio-TLC. In the examined substrate concentration range, the signal intensity was continually increased up to a high concentration of E. coli malic-kemptide fusion protein as determined by the radio-TLC. The use of this detection method facilitates a rapid data analysis with a high sensitivity.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동