Protein immobilization is a key step for the development of protein biosensor. In recent years, people have been seeking various immobilization ways aiming at satisfying a number of standards for excellent performance of a biosensor, among which, gold binding polypeptide (GBP) based immobilization began to attract people’s attention. Here, we established a universal detecting platform utilizing the GBP, protein A (SpA) and protein G (SpG). Kinds of GBP-fusion protein, such as GBP-SpA, SpA-GBP, GBP-SpG and SpG-GBP, were prepared via recombinant DNA technology. Surface plasmon resonance (SPR) was applied to investigate the affinity of these proteins with the gold surface as well as with immunoglobins. Furthermore, electrochemical impedance spectroscopy was analyzed for the detection optimization of hepatitis B viral surface antigen [Our work was supported in part by the IT Leading R&D Support Project from the Ministry of Knowledge Economy through IITA.].
키워드
Gold binding polypeptideHepatitis B virus detectionSurface Plasmon resonanceElectrochemistry
저자
Tae Jung PARK [ BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, and Center for Ultramicrochemical Process Systems, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701. ]
Shun ZHENG [ Department of Chemical & Biomolecular Engineering (BK21 program), KAIST ]
Sang Yup LEE [ 2Department of Chemical & Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, Center for Ultramicrochemical Process Systems, Department of Bio & Brain Engineering, Department of Biological Sciences, and BioInformatics Research Center, KAIST ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동