A sensitive protease assaying method is key tool for elucidating protease function, and developing therapeutics because of implications in the pathogenesis of diverse disease such as viral and bacterial infections and cancer and neurodegenerative diseases. Also it has been reported that secreted amount of proteases are related to cancers. Activity of proteases is assayed by measuring amount of digested substrates. Due to feasibility for developing high throughput assaying formats, synthetic short peptides are preferred. For developing fast and sensitive protease assaying methods, also nanoparticles such as quantum dots, gold nanoparticles and magnetic nanoparticles have been utilized. The reported methods show relatively low detection limits. However still these techniques require long assaying times(more than 12 hours) for acquiring enough detecting signals. Therefore, I propose a more rapid and sensitive way to assay activity of protease by incorporating a signal amplification method with gold nanoparticle and RNase H. More detail working principle and detection limit of our protease assaying method will be presented
키워드
Protease activity assayPeptideRNase HGold nanoparticleFluoresence Energy Transfer
저자
JOONG HYUN KIM [ BioNano Research Center, KRIBB, Daejeon, 305-806. ]
BONG HYUN CHUNG [ BioNano Research Center, KRIBB, Daejeon, 305-806. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동