Earticle

초록

영어

Insect cell lines have been used extensively for the production of numerous functional eukaryotic proteins. However, insect cell systems have limited commercial use due to a lack of complex glycosylation.
Drosophila S2 cells have been developed as a plasmid-based and nonlytic expression system using recombinant baculovirus, infects into insect cells and does not require any selection markers. A gene encoding human erythropoietin (hEPO) or human 90K (h90K) fused with a hexa-histidine (His6) tag under the control of the Drosophila originated metallothionein promoter (pMT) was stably transfected into Drosophila S2 cells. In the preliminary studies, we found that expressed proteins had simple N-glycan structures instead of complex
structures. We thought that this result caused by N-acetylglucosaminidase (GlcNAcase) through enzyme assay experiment. Therefore, this research will propose that suppression of GlcNAcase and multiple expression of N-glycosyltransferases using recombinant baculovirus
strategy.

키워드

저자

• Kyoung Ro KIM [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH ]
• Dong Gyun KANG [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH ]
• So Young JANG [ Mass Spectrometry Research Team, Korea Basic Science Institute, Ochang, 363-883. ]
• Young Hwan KIM [ Mass Spectrometry Research Team, Korea Basic Science Institute, Ochang, 363-883. ]
• Hyung Joon CHA [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576