Dengue virus (DENV) spread by female mosquitoes, specifically the Aedes aegypti species is an infectious viral disease endemic to tropical areas worldwide. Currently four serotype (DEN1-4) of DENV exist. To date, it is estimated that two-fifths of the world’s population are at risk for being exposed to at least one serotype of DEN1-4(WHO,2002). No dengue vaccine is available yet. Recently, however, attenuated candidate vaccine viruses have been developed. This research was also being conducted to develop recombinant edible vaccine expressed in rice cell system. E glycoprotein has a major role in host cell attachment and entry and is recognized as the primary antigen that induces protective immunity. The binary expression vector, pMYN81, containing E glycoprotein gene was constructed, which was designed with RAmy3D promoter which is well known as a strong promoter under sugar starvation. Putative transformed calli were selected by genomic DNA PCR analysis after introducing into the nucleus of the rice cell by gene gun. The expression levels of mRNA and E glycoprotein were confirmed using Northern blot analysis and Western blot analysis.
키워드
Dengue virusricevaccine
저자
Kim MI-YOUNG [ Dept. of Biological Sciences, Chonbuk National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동