Earticle

초록

영어

Transgenic rice, Oryza sativa L., cells were cultured to produce human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig), a fusion protein used for the treatment of rheumatoid arthritis. This plant cell culture system with the signal peptide for the secretion of target
protein into the extracellular medium and the inducible RAmy3D promoter was used for the production of the recombinant therapeutic protein in order to overcome the constraint of low production level.1) However, the RAmy3D promoter system has a drawback in decreasing
cell viability because the promoter is induced only by sugar starvation. The cell death stimulated the release of protease, which degraded target protein.2) In order to solve these instability problems of the product after secretion, in situ product recovery was performed by using a modified flask.3) The modified flask was specially designed with glass filter to make medium exchange easy and successful. The medium exchange provided an improved culture condition for the cells because proteases could be removed from the culture environment by dilution with new medium after exchange. Contrary to the expectation, it was found that the production level of hCTLA4Ig was not increased despite of the better maintenance of cell mass than those of control. These results show that in situ product recovery using a modified flask could not enhance the production of hCTLA4Ig in transgenic rice cell culture system.

키워드

저자

• Boreum YUN [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Jun-Young KWON [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Hye-Ran LEE [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Ji-Yeon HAN [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Mi-Hee YOO [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Ji-Won CHOI [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Yong Suk YANG [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Dong-Il KIM [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576