Magnetic nanoparticles isolated from Magnetospirillum sp. AMB-1 can be applied to various therapeutic materials. However, the intracellular uptake pathway of magnetic nanoparticles has not been clearly elucidated yet. In this study, we investigated the intracellular uptake mechanism of magnetic nanoparticles. Magnetic nanoparticles were delivered to the endothelial progenitor cells (EPC) and their uptake pathways were investigated through various methods. Magnetic nanoparticle internalization into EPCs increased with incubation time in the presence of magnetic nanoparticles in the medium. Cellular uptake of magnetic nanoparticles was inhibited in the low temperature (4℃) and confocal study demonstrated that nanoparticles were internalized via endosome at least within 1 h after incubation. We also studied the exocytosis of magnetic nanoparticles by time-dependent manner. Our results demonstrated that the uptake process of magnetic nanoparticles was dynamic and the magnetic nanoparticles from AMB-1 could be internalized into the EPCs through energy-dependent endocytotic pathway.
키워드
Magnetic nanoparticleEndocytosisCellular uptakeEndothelial progenitor cell
저자
Jeong Ah KIM [ School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea. ]
Hong Jai LEE [ School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea. ]
Tai Hyun PARK [ School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동