Earticle

초록

영어

A method for measuring the ethanol concentration in a yeast culture broth was developed using both microtubes and a 96-deepwell microplate. The strategy involved first the solvent extraction of ethanol from the yeast culture broth and measurements of the ethanol concentration using the dichromate oxidation method. Particular focus was made on selecting the extraction solvent as well as determining the measurable range of ethanol concentrations using this solvent extraction-dichromate oxidation method. This method was developed as an assay format in 2.0 ml microtubes and 1.2 ml 96-deepwell microplates, and the ethanol concentration in the batch cultures and fed-batch fermentations was measured. Trin-butyl phosphate (non-alcoholic solvent, density=0.9727, solubility in water=0.028% (w/v)) was used for solvent extraction when measuring the ethanol concentration from the yeast culture broth. The maximum detectable ethanol concentration was 8% (v/v) when 10 g potassium dichromate in 100 ml of 5M sulfuric acid was used. The concentrations determined from the solvent extractiondichromate
oxidation methods were remarkably similar to those from gas chromatography, in which samples were prepared from seven experiments, such as four batch cultures and three fed-batch fermentations.

키워드

저자

• Hyun-Beom SEO [ Division of Food and Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701, ]
• Kyung-Hwan JUNG [ Division of Food and Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701, ]
• Ji-Hye HA [ 1College of Bioscience and Biotechnology,Kangwon National University, Chuncheon 200-701, Republic of Korea. ]
• Hyeon-Youg LEE [ Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576