High-level production of ethanol during fed-batch ethanol fermentation with a control of aeration rate and nonsterile glucose powder feeding of Saccharomyces cerevisiae
Hyun-Beom SEO, Kyung-Hwan JUNG, Jae-Gun HAN, Hyeon-Yong LEE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A105284
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원문정보
초록
영어
In this study, we utilized a unique strategy for the fed-batch fermentation process using ethanol-tolerant Saccharomyces cerevisiae to achieve a high-level of ethanol production that could be practically applied to industrial ethanol production. During this study, the aeration rate was controlled at 0.0, 0.13, 0.33, and 0.8 vvm to determine the optimal aeration conditions for the production of ethanol. Additionally, nonsterile glucose power was fed during fed-batch ethanol fermentation, and the amount of corn-steep liquor (CSL) in the medium was optimized. When aeration was conducted, the ethanol production and productivity were superior to those when the aeration was not conducted. Specifically, the maximum ethanol production reached approximately 160 g/L ethanol when the reaction was aerated at 0.13 vvm. Ethanol productivity during the early phase of the process increased, then decreased gradually during the later phase. Despite this finding, when aeration was conducted, the overall ethanol yield was not superior to that obtained when aeration was not conducted. In addition, ethanol consumption was observed at aeration rates of 0.3 and 0.8 vvm which resulted in a remarkable decrease in ethanol productivity and yield. However, the use of a much more inexpensive C-source may enable the process to be directed toward improvement of the overall ethanol production and productivity in a process that is aerated at 0.13 vvm. Furthermore, if a repeated fed-batch process in which the withdrawal and fill is conducted prior to 36 hr can be employed, aeration at a rate of 0.33 and/or 0.8 vvm may improve the overall ethanol productivity. Finally, the overall ethanol productivities in this study varied throughout the fed-batch ethanol fermentation, which indicates that the optimal operational strategy for achieving improved ethanol productivity may be set-up according to various aeration rates.
Hyun-Beom SEO [ Division of Food and Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701, Republic of Korea. ]
Kyung-Hwan JUNG [ Division of Food and Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701, Republic of Korea. ]
Jae-Gun HAN [ Division of Biomaterials Engineering, Kangwon National University, Chuncheon 200-701, Republic of Korea. ]
Hyeon-Yong LEE [ 1)Division of Biomaterials Engineering 2)Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동