Jian QI, Jung Hee PARK, Woo Seok CHOE, Ik-Keun YOO
언어
영어(ENG)
URL
https://www.earticle.net/Article/A105260
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원문정보
초록
영어
Conventional biopanning protocol has been applied to explore the peptide sequence having affinity to a target compound starting from a phage-displayed peptide library through a batch type adsorption protocol. The basic principal of biopanning protocol is very similar to the general chromatographic separation which is widely used in bioseparation processes. This study was carried out to improve the efficiency of biopanning protocol based on that similarity of both protocols. The biopanning protocol was automated by using a FPLC equipped with monolithic column which virus particles pass through. This new protocol made it possible to develop “chromatographic library panning protocol” which can search for the specific peptide sequence within a short time compared to the conventional biopanning protocol. Pb2+ has been known to be highly toxic to a majority of living entities and thus classified with other heavy metal ions as endocrine disruptors. The iterative passing of phage particles through the Pb2+-combined monolithic column in FPLC was done to find the peptide sequence having high affinity to Pb2+. Then the screened Pb2+- binding phage particles was sequentially loaded to the other monolithic columns combined with other metal ions (Ni2+, Cu2+, Zn2+, Co2+) to remove the peptide sequence having cross-binding affinity to other metal ions.
키워드
biopanningFPLCPb2+
저자
Jian QI [ Dept. of Chemical Eng., Sungkyunkwan University. ]
Jung Hee PARK [ School of Chemical Eng. & Bioeng., University of Ulsan. ]
Woo Seok CHOE [ Dept. of Chemical Eng., Sungkyunkwan University ]
Ik-Keun YOO [ School of Chemical Eng. & Bioeng., University of Ulsan. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동