Earticle

초록

영어

We previously reported the novel β-glucosidase from Shinorhizobium meliloti that had distinct activity to the synthetic indican or plant extracts of Polygonum tinctorium. However, the Shinorhizobium β-glucosidase showed a relatively lower activity for indican and also mainly found in insoluble fraction when expressed solely without any tag or fusion partner, although it showed a quiet high activity toward MUG (4-Methylumbeliferyl-β-D-glucopyranoside) and X-gal in the screening procedures. These shortcomings of the enzyme were needed consideration to screen another enzyme with more high activity toward the target substrate indican or indican-containing plant extract. We, therefore, further attempted to screen the related enzyme from various sources of microbe minded in previous work. Resultantly, we found a potential enzyme with high activity to indican, from A. tumefaciens and then expressed functionally in E. coli. In this presentation, we characterized the enzyme in detail and further analyzed the potential of the enzyme in terms of expression level, solubility and specific activity for practical application of indigo production.

키워드

저자

• Ji-Yun KIM [ Department of biological sciences, College of natural science, Chonnam national university. ]
• Youn-Sook SHINA [ Department of Clothing and Textiles, Chonnam national university. ]
• Geun-Joong KIM [ Department of biological sciences, College of natural science, Chonnam national university. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576