Earticle

초록

영어

Clostridium acetobutylicum is one of the major acetone-butanolethanol (ABE) producers. Cell density of C. acetobutylicum could relate to several factors including, nutrients, substrate inhibition, and limited heat dissipation, etc. To improve cell density, it was necessary; to design a balanced nutrient medium that contains all the necessary components for supporting cell growth, while avoiding inhibition. The maximum cell density at 600 nm (MaxOD600) was measured in order to determine the nutrient balance of each component. The cells were cultured at 37°C in the test tube containing varying concentration of component, anaerobically. From the results, the highest MaxOD600, 12.5, which was 1.8 times higher than that obtained at clostridial growth media (CGM), was obtained from the modified medium, designated C. acetobutylicum media 1 (CAM1). Batch-fermentations in CAM1 were carried out, and the cell concentrations increased gradually through cultivation up to OD600 values of 19.7, 23.7, and 18.6, which were 1.8, 2.6, and 1.4 times higher than those obtained in CGM, by ATCC 824, M5, and M5E1AB strains, respectively. [This work was supported by the Ministry of Knowledge Economy grant funded by the Korea government (10030795). Further supports by the GS-Caltex and the BioFuelChem].

키워드

저자

• Jaeeun OH [ Department of Chemical and Biomolecular Engineering, KAIST, ]
• Jin Young LEE [ Department of Chemical and Biomolecular Engineering, KAIST, ]
• Sang Yup LEE [ Department of Chemical and Biomolecular Engineering, KAIST, ]
• Yu-Sin JANG [ Department of Chemical and Biomolecular Engineering, KAIST, ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576