Elimination of By-Products Formation during Production of 1,3-Propanediol from Glycerol in Klebsiella Pneumoniae by Inactivation of the Oxidative Pathway for Glycerol Metabolism
Mi-Young SEO, Jeong-Woo SEO, Sun-Yeon HEO, Jin-Oh BAEK, Dina RAIRAKHWADA, Baek-Rock OH, Pil-Soo SEO, Min Ho CHOI, Chul Ho KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A104884
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원문정보
초록
영어
Klebsiella pneumoniae is a typical microbial strain capable of producing 1,3-propanediol that is a valuable chemical that is used mainly for the synthesis of polymethylene terephalates by polymerization with terephthalates (1). The microbial production of 1,3-PD by K. pneumoniae involves the formation of various by-products which are synthesized through the oxidative pathway. 2,3-Butanediol, one of the main by-products, may serve as an obstacle for obtaining a high purity of 1,3-PD in downstream processes because of its similar boiling point (2). Hence, to eliminate the by-products synthesis, the oxidative branch of glycerol metabolism was inactivated by deleting from the chromosomal DNA the genes involved in the synthesis of by-products. The by-product formation except for acetate was eliminated in the resultant strains, giving the production yield of 0.57 mol/mol which was higher as compared to that of the wild type strain which gave the production yield of 0.47 mol/mol.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동