Earticle

초록

영어

Previously, Streptomyces interspecies DNA microarray analysis was successfully used to detect global changes in mRNA abundance associated with doxorubicin overproduction in S. peceutius, revealing wblA (SCO3579) as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces species (Kang et al, 2007, J. Bacteriol.). Further analysis revealed another potential down-regulator in antibiotic biosynthesis, a tetR-family transcriptional regulator gene, SCO1712. Adventitious expression of SCO1712 reduced doxorubicin production in S. peucetius as well as two major S. coelicolor antibiotics, actinorhodin (ACT) and undecylprodigiosin (RED). The SCO1712 gene disruption from the S. coelicolor chromosome also resulted in a noticeable increase of both antibiotic productions. Moreover, transcripts encoded by major pathway-specific antibiotic activators in S. coelicolor (i.e., actII-ORF4 for ACT, redD/Z for RED, and cdaR for CDA) were stimulated in the SCO1712-disrupted S. coelicolor mutant, suggesting that SCO1712 acts broadly to down-regulate antibiotic biosynthesis in Streptomyces species. Interestingly, a previously-identified down-regulator wblA transcript was also stimulated in the SCO1712-disrupted S. coelicolor mutant, implying that the SCO1712 might down-regulate wblA expression as well as yet-unidentified antibiotic positive regulatory gene(s) which should bypass the wblA-dependent antibiotic down-regulatory system in S. coelicolor.

키워드

저자

• Han-Na LEE [ Dept. of Biological Engineering, Inha University, ]
• Jun-Hee NOH [ Dept. of Biological Engineering, Inha University, ]
• Eung-Soo KIM [ Dept. of Biological Engineering, Inha University, ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576