Narayan Prasad NIRAULA, Tae Jin OH, Jae Kyung SOHNG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A104874
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원문정보
초록
영어
Genome of Streptomyces peucetius ATCC 27952 contains 23 cytochrome p450 genes. The ability of the cytochrome P450 heme monooxygenases to catalyze difficult oxidation reactions, often with high specificity and selectivity, makes them attractive for numerous biotechnological applications. However they are generally limited by low turnover rates and low stability, and their minimum requirements for catalysis include a cofactor as source of electrons (NAD(P)H), partner proteins for electron transfer, and dioxygen. Cytochrome P450 used in this study to investigate the hydroxylating property expressing in Escherichia coli belongs to the CYP105P2 family from S. peucetius. Owing to the inactivity of the isolated p450 enzymes,coexpression of three-component electron transfer chain in Escherichia coli was done from two plasmids with different selection markers and compatible origins of replication. The enzyme was supported in its activity with putidaredoxin reductase (CamA) and putidaredoxin (CamB) from Pseudomonas putida, supplying this enzyme with the reducing equivalents necessary in class I type electron transfer system. Hydroxylation of flavone ring was achieved as indicated by HPLC/GC-MS analysis.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동