Earticle

초록

영어

From the cells of Bacillus lichemiformis which can degrade cellulose or other cellulosic materials, the putative cellulase gene, gene ID BLi01882, was cloned. For cell surface display of cellulase, the gene was inserted into the pET21a E. coli vector containing Ice Nucleation Protein (INP) gene as an anchor linker. The INP gene, which is expressed as an outer membrane protein, was obtained from Pseudomonas syringae. The ultimate purpose of artificial surface display is to perform saccharification and fermentation processes simultaneously at a step. Thus, expense and time for production of ethanol can be reduced. In this study, two different cloned vectors, with and without INP gene, were compared in respect of enzyme activity. Activities of the cellulases made, respectively, from two vectors were compared with the positive control using Congo red staining method. The clear zones of cloned products were smaller than the positive control, because it was thought that the cloned products have only one cellulase gene whereas the positive control has more than three kinds of cellulases. When two cloned products, with and without INP gene, were expressed, their enzyme activities did not make a difference on both Congo red staining and SDS-PAGE. It indicates that the fusion of INP gene did not affect either enhancement or inhibition of enzyme expression.

키워드

저자

• Dae Hwan KIM [ Dept.of Biotechnology, Yonsei Universiy, Seoul, 120-749. ]
• Heeyong JUNG [ Dept.of Biotechnology, Yonsei Universiy, Seoul, 120-749. ]
• Chul Soo SHIN [ Dept.of Biotechnology, Yonsei Universiy, Seoul, 120-749. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576