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초록

영어

than those of daidzein. P450s have the ability of converting daidzein to hydroxydaidzein i.e. 3΄-hydroxy, 6-hydroxy, and 8-hydroxydaidzein. We already found that CYP105D7 (SAV7469) has the specific activity of converting daidzein to hydroxydaidzein. We compared the various strains for the biosynthesis of hydroxydaidzeins. Among strains examined, Streptomyces avermitilis MA4680 highly converted daidzein to orthospecific dihydroxyisoflavones. We investigated recombinant Streptomyces avermitilis strain found to have 3.6 times higher ability than wild type to convert 3΄-hydroxydaidzein. In this report, we found a new pathway-specific regulator which binds to the promoter region of CYP105D7 (SAV7469) in Streptomyces avermitilis by the DNA affinity capture assay method. Since this regulator is well conserved throughout the Streptomyces species, it is expected to have an important role in Streptomyces avermitilis. We made an overexpression mutant, deletion mutant of this regulator and checked the activity of converting daidzein to hydroxydaidzein compared to the wild type strain.

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저자

• Ji-Nu KIM [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744. ]
• Eunjung SONG [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744. ]
• Eun-Jung KIM [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744. ]
• Bo-Rahm KIM [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744. ]
• Nahum LEE [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744. ]
• Byung-Gee KIM [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576