Earticle

초록

영어

Elevated total homocysteine (Hcy) levels have been associated with an increased risk of atherosclerotic disease, coronary heart disease, and clinical stroke. Cysteine (Cys) can be obtained as the final product of the transsulfuration pathway through Hcy metabolism. It is essential and important to develop some pre-column fluorescent derivatizing reagents for the detection of the Hcy and Cys with ease, quick, accuracy and specificity. 4-maleimide-7-nitro-2,1,3-benzoxadiazole (NBD-M), a novel pre-column fluorescent derivatizing reagent was designed and synthesized. NBD-M was synthesized on the base of bezofurazan skeleton. Maleimide and 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) were dissolved in the acetonitrile solution, respectively. NBD-Cl solution was added drop-wise to the maleimide solution containing sodium carbonate and was reacted at room temperature for 30 minutes. And then the product was separated by silica gel chromatography and determined by mass spectroscopy. NBD-M has relative low molecular weight and reacted with Hcy and Cys selectively. The high stabilities of the derivatives of Hcy and Cys with NBD-M are suitable for the HPLC analysis.

키워드

저자

• Kwang-Hwan JHEE [ Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701. ]
• Lu-An FAN [ Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701. ]
• Seun-Ah YANG [ The Center for Traditional Microorganism Resources, Keimyung University, Daegu, 704-701. ]
• In-Seon LEE [ The Center for Traditional Microorganism Resources, Keimyung University, Daegu, 704-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576