Earticle

초록

영어

In this study, a methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis and its improvement by metabolic engineering technology are demonstrated. Two amino acids, cysteine and methionine, and four vitamins, nicotinic acid, pyridoxine HCl, thiamine, and Ca-pantothenate, were identified as non-substitutable essential compounds to be supplemented to a minimal medium for the sustainable growth of Mannheimia succiniciproducens by the genome-based in silico analysis. The in silico predictions were confirmed by cultivating the Mannheimia succiniciproducens on a CDM containing the six nonsubstitutable essential compounds, and it was further verified by observing no cell growth on the CDM lacking any one of the non-substitutable essentials. To enhance the cell growth and the succinic acid production, as a target product, an optimal CDM was developed with a single-addition technique. The fermentation on the optimal CDM increased the succinic acid productivity by 36%, the final succinic acid concentration by 17%, and the succinic acid yield on glucose by 15% compared to the cultivation using a complex medium. Moreover, metabolic engineering technologies were applied to improve the Mannheimia succiniciproducens for the further enhancement of CDM in an economical aspect.

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저자

• Yong J CHOI [ Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea1Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]
• Hyohak SONG [ Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]
• Tae Yong KIM1, [ Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]
• Bo-Kyeong CHOI [ 2BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea. ]
• Seong Jun CHOI [ 3Metabolic and Biomolecular Engineering National Research Laboratory, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea. ]
• Lars K NIELSEN [ 4Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland 4072, Australia. ]
• Ho NAm CHANG [ Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea1Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]
• Min-Sun HAN [ Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea1Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]
• Sang Yup LEE [ Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea1Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576