Byoung Hoon YOON, Woo Young JEON, Woo Yong SHIM, Jung Hoe KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A104811
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원문정보
초록
영어
Xylose reductase (XR) is a key enzyme in D-xylose metabolism, catalyzing the reduction of D-xylose to xylitol. Expression of XR in Candida tropicalis is significantly repressed in cells grown on glucose by catabolic repression. This is one of the reasons that glucose cannot be used as a cosubstrate in xylitol production. A gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was isolated from the genomic DNA of C. tropicalis ATCC 20913 to construct a constitutive expression cassette for this strain. The endogenous XR gene (xyl1) was placed under the control of GAPDH promoter and integrated into the genome of C. tropicalis N43 which is xyl2-disrupted and xyl1-partially disrupted mutant. Expression of XR was confirmed by determining enzyme activity in cells grown on a medium containing glucose as a carbon source. The resulting recombinant yeast, C. tropicalis JA4, showed higher XR activity (550mU/mg of proteins) than that of the parental strain (18mU/mg of proteins). Batch culture was performed for xylitol production with JA4 using glucose as a cosubstrate. The initial xylitol production rate of JA4 was higher than that of the parental strain.
키워드
XylitolXylose reductaseCandida tropicalis
저자
Byoung Hoon YOON [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
Woo Young JEON [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
Woo Yong SHIM [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
Jung Hoe KIM [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동