Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] can be tailored to achieve a variety of polymeric materials, from hard crystalline plastics to very elastic rubber by varying 4HB monomer composition. In general, 3HB is produced by feeding precursor carbon sources such as glucose, fructose, sucrose and plant oil, while 4HB is produced from precursors such as γ-butyrolactone, 1,4-butanediol and 4-hydroxybutyric acid. Plant oils are desirable feedstocks for PHA production because they are inexpensive carbon sources. The theoretical yield coefficients of PHA production from plant oils are as high as over 1.0 g-PHA per g-plant oils used, since they compose a much higher number of carbon atoms per weight. In this study, we attempted to produce P(3HB-co-4HB) copolymer by Ralstonia eutropha ATCC 17699 using a mixture of soybean oil and γ-butyrolactone. The effect of soybean oil and γ-butyrolactone on cell growth was investigated by varying the concentrations in flask cultures. The effects of culture volume on cell growth and P(3HB-co-4HB) production were also carried out. In addition, the 4HB precursor feeding strategies were studied to improve the copolymer production.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
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