Embryonic stem cells (ESCs) have enormous potential for satisfying the demand for transplantable cells, once we learn how to properly control their proliferation and differentiation into specific cell types. We explore the use of a microfluidic platform to deliver growth factors to ESCs in a controlled manner to direct cell proliferation and differentiation. In order to examine the effect of BMP signaling on ESC proliferation and differentiation, a mouse ESC line (BRE mESC) harboring a BMP responsive element (BRE) driving lacZ expression was generated. When BRE-mESCs were grown in the conventional Petri dish culture condition (with non-circulating media), ESCs respond heterogeneously to BMP signaling irrespective of the presence or absence of LIF. However, a significantly improved uniform response to BMP4 treatment was observed in the microfluidic chamber where steady levels of BMP4 were constantly perfused over ESCs. This suggests that perfusion of a growth factor to ESCs may be an efficient way to direct the differentiation of ESCs, and this approach may be generally applicable to expand the population and uniformly steer the differentiation of adult stem cells.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동