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For efficient saccharification from alginate, a marine bacterium was isolated from seawater near the Korean south coast. Based on 16S rDNA sequence, the isolated strain was identified as Pseudoalteromonas agarovorans. Various environmental factors affecting saccharification of alginate using P. agarovorans CHO-12 have been investigated in flask cultures. The optimum concentration of sugar was obtained at 30 rpm and 29oC. Among various NaCl concentrations, when NaCl concentration was increased from 10g/l to 30g/l, the cell concentration sharply increased, while there is no increase at above 40g/l. The maximum sugar concentration was obtained at 13.8g/l when 30g/l of NaCl was used. Yeast extract and CSL were the best nitrogen source for efficient saccharification. Especially, the sugar concentration of 14.9 g/l was obtained after 3 days of culture using a mixture of 1.0 g/l of yeast extract and 1.5 g/l of peptone. Scale up was carried out at 50l of reactor for 3 days using P. agarovorans CHO-12 and Stenotrophomonas maltophilia sp. When S. maltophilia was used, cell concentration was about 2.0 fold higher than that of P. agarovorans CHO-12. On the other hand, when P. agarovorans CHO-12 was used, the maximum saccharification rate was obtained, 7.5 g/l/day after 2 days of culture, which was about 10.0 fold higher than that of S. maltophilia.

키워드

저자

• Da Eun KIM [ Department of Polymer Science & Engineering, Chosun University ]
• Hoon CHO [ Department of Polymer Science & Engineering, Chosun University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576